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proA
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proA

glutamate-5-semialdehyde dehydrogenase, required for normal and osmoadaptive proline biosynthesis
locus
BSU_13130
pI
5.10
mw
45.17 kDa
protein length
415 aa Sequence Blast
gene length
1248 bp Sequence Blast
function
biosynthesis of proline
product
glutamate-5-semialdehyde dehydrogenase
essential
no
ec
1.2.1.41
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]

    • Gene

    Coordinates
    1,379,605 1,380,852

    Phenotypes of a mutant

  • auxotrophic for proline [Pubmed|21784929]

    • The protein

    Catalyzed reaction/ biological activity

  • L-glutamate 5-semialdehyde + NADP+ + phosphate --> H+ + L-glutamyl 5-phosphate + NADPH (according to UniProt)

    • Protein family

  • gamma-glutamyl phosphate reductase family (single member, according to UniProt)

    • Structure

  • [PDB|1O20] (from ''Thermotoga maritima'', 46% identity, 67% similarity) [Pubmed|14705032]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|5592C5163CF024289B850916516E2C8C7AD43136|proB]-[gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA]
    description
    [Pubmed|21233158]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21233158], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|T-box|T-box]: anti-termination, in [regulon|T-box|T-box]
  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR]: regulation, [pubmed|30355672], in [regulon|F4097349A563503468A2A14F062AEAC532C7917A|YlxR regulon]

    • regulation

  • induced by proline limitation ([SW|T-box]) [Pubmed|21233158]
  • expression requires functional [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR] [pubmed|30355672]

    • additional information

  • [protein|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|ProA] is absent from the membrane proteome of a '[protein|A01637E1BFABA20A2923BA85A25CCD8A0D665F78|BdbC]-[protein|CED818CEF5B573A40F382157375E60C0B331F098|BdbD]' mutant [PubMed|22540663]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
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    Biological materials

    Mutant

  • BKE13130 ([gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AACTTCACTCATTTTTCCGC, downstream forward: _UP4_TAGCGGGGTAATGTTCAATG
  • BKK13130 ([gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AACTTCACTCATTTTTCCGC, downstream forward: _UP4_TAGCGGGGTAATGTTCAATG

    • References

    Reviews

  • 25367752

    • Original publications

  • 19258532,21233158,22540663,21784929,17562291,23869754,25344233,28752945,30355672