SubtiBank SubtiBank
mleA [2019-02-19 18:23:56]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

mleA [2019-02-19 18:23:56]

malic enzyme
Locus
BSU23550
Isoelectric point
4.89
Molecular weight
45.73 kDa
Protein length
439 aa Sequence Blast
Gene length
1320 bp Sequence Blast
Function
malate utilization
Product
NAD-dependent malate dehydrogenase
Essential
no
E.C.
1.1.1.38
Synonyms
yqkJ

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
2,451,463 2,452,782

The protein

Catalyzed reaction/ biological activity

  • (S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate - pyruvate
  • Protein family

  • malic enzymes family (according to Swiss-Prot)
  • Paralogous protein(s)

    Domains

  • ACT domain (aa 9 ... 84) (according to the Interpro database)
  • Structure

  • 2A9F (from Streptococcus pneumoniae, 42% identity from aa 90 - 435)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • AnsR: repression, PubMed, in AnsR regulon
  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • ansA: expressed in the presence of asparagine (AnsR) PubMed
  • view in new tab

    Description

    Regulation

  • ansA: expressed in the presence of asparagine (AnsR) PubMed
  • view in new tab

    Biological materials

    Mutant

  • MGNA-C412 (yqkJ::erm), available at the NBRP B. subtilis, Japan
  • GP1136 (cat) available in Stlke lab
  • BKE23550 (mleA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC
  • BKK23550 (mleA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC
  • References

    Loading