mleA [2019-02-19 18:23:56]

malic enzyme

Locus: BSU23550

Isoelectric point: 4.89

Molecular weight: 45.73 kDa

Protein length: 439 aa

Gene length: 1320 bp

Function: malate utilization

Product: NAD-dependent malate dehydrogenase

Essential: no

E.C.: 1.1.1.38

Synonyms: yqkJ

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids

Gene

Coordinates: 2,451,463 2,452,782

The protein

Catalyzed reaction/ biological activity

• (S)-malate + NAD⁺ = pyruvate + CO₂ + NADH (according to Swiss-Prot) malate - pyruvate

Protein family

• malic enzymes family (according to Swiss-Prot)

Paralogous protein(s)

• YtsJ, MalS

Domains

• ACT domain (aa 9 ... 84) (according to the Interpro database)

Structure

• 2A9F (from Streptococcus pneumoniae, 42% identity from aa 90 - 435)

Expression and Regulation

Operons

• Genes: mleN-mleA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • AnsR: repression, PubMed, in AnsR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • ansA: expressed in the presence of asparagine (AnsR) PubMed

• Genes: ansA-ansB-mleN-mleA
  Description: PubMed

  Regulation

  • ansA: expressed in the presence of asparagine (AnsR) PubMed

Biological materials

Mutant

• MGNA-C412 (yqkJ::erm), available at the NBRP B. subtilis, Japan
• GP1136 (cat) available in Stlke lab
• BKE23550 (mleA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC
• BKK23550 (mleA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC

References

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