ktrC [Fri Dec 09 2016 17:26:47 GMT+0100 (CET)]

low affinity potassium transporter KtrC-KtrD, peripheric membrane component

locus: BSU14510

pI: 5.00

mw: 24.00 kDa

function: potassium uptake

product: low affinity potassium transporter KtrC-KtrD,
peripheric membrane component (proton symport)

essential: no

synonyms: ylxV, yzaC, ykqB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

[SW|Categories] containing this gene/protein

• [SW|transporters/ other], [SW|metal ion homeostasis (K, Na, Ca, Mg)], [SW|coping with hyper-osmotic stress], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence): 1,520,531 -> 1,521,196

The protein

Paralogous protein(s)

• [protein|search|KtrA]

[SW|Domains]

• contains a [SW|RCK_N domain] at the N-terminus (according to [http://www.uniprot.org/uniprot/?query=domain:RCK N-terminal domain* UniProt])
• contains a c-di-AMP-binding [SW|RCK_C domain] at the C-terminus (according to [http://www.uniprot.org/uniprot/?query=domain:RCK C-terminal domain* UniProt]) [Pubmed|23671116]

Effectors of protein activity

• the protein binds c-di-AMP [Pubmed|23671116]

Structure

• [PDB|4J7C] (the [protein|search|KtrA]-[protein|search|KtrB] complex, 56% identity) [Pubmed|23598340]
• [PDB|4XTT] (the ''S. aureus'' KtrA [SW|RCK_C domain] in complex with c-di-AMP, 57% identity) [Pubmed|25957408]

[SW|Localization]

• peripheral membrane protein [Pubmed|12562800]

[SW|Interactions]

• [protein|search|KtrC]-[protein|search|KtrD] [Pubmed|12562800]

Expression and Regulation

Operon

• ''ktrC'' [Pubmed|12562800]

Regulation

• constitutively expressed [Pubmed|12562800]

Biological materials

Mutant

• GHB6 (''ktrC''::''spec''), [Pubmed|12562800] (available in [SW|Erhard Bremer]'s and [SW|Jörg Stülke]'s) labs, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A955&Search=1A955 BGSC] as 1A955
• GP2264 (''[protein|search|ktrC]''::''ermC''), available in [SW|Jörg Stülke]'s lab
• GP2079 (''[protein|search|ktrC]''::''tet''), available in [SW|Jörg Stülke]'s lab
• GP2083 (D(''[protein|search|ktrA]-[protein|search|ktrB]'')::''aphA3'' D''[protein|search|ktrC]''::''tet''), available in [SW|Jörg Stülke]'s lab

Expression vector

• pGP2907 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

References

Reviews

• 25869574

Original publications

• 12562800,23671116,23598340