ktrC

glutamate-controlled potassium channel KtrC-KtrD, peripheric membrane component

Locus: BSU_14510

Isoelectric point: 5.63

Molecular weight: 24.20 kDa

Protein length: 221 aa

Gene length: 666 bp

Function: potassium uptake

Product: glutamate-controlled potassium channel KtrC-KtrD, peripheric membrane component

Essential: no

Synonyms: ylxV,yzaC,ykqB

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Metal ion transporter
• Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Potassium uptake/ export
• Information processing → Targets of second messengers → Targets of c-di-AMP
• Lifestyles → Coping with stress → Coping with hyper-osmotic stress
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,520,531 1,521,196

The protein

Protein family

• KtrA potassium transport family (with KtrA, according to UniProt)

Paralogous protein(s)

• KtrA

Kinetic information

• the KtrC-KtrD channel has a low affinity for potassium, this is determined by KtrD PubMed
• the affinity of KtrC-KtrD for potassium is strongly increased in the presence of glutamate (from 0.278 mM to 0.17 mM) PubMed

Domains

• contains a RCK_N domain at the N-terminus (aa 4-126)(according to UniProt)
• contains a c-di-AMP-binding RCK_C domain at the C-terminus (aa 135-219) (according to UniProt) PubMed

Effectors of protein activity

• binds ADP and ATP PubMed
• the protein binds c-di-AMP, K_D = 30 nM, this results in inhibition of potassium uptake PubMed
• the affinity of KtrC-KtrD for potassium is strongly increased in the presence of glutamate PubMed

Structure

• 4J7C (the KtrA-KtrB complex, 56% identity) PubMed
• 6I8V (KtrC in complex with ATP) PubMed
• 4XTT (the S. aureus KtrA RCK_C domain in complex with c-di-AMP, 57% identity) PubMed

Localization

• peripheral membrane protein PubMed

Expression and Regulation

Operons

• Genes: ktrC
  Description: PubMed

  Regulation

  • constitutively expressed PubMed

• Genes: kinC-ykqA-ktrC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon

  Regulation

  • constitutively expressed PubMed

Biological materials

Mutant

• MGNA-A904 (ykqB::erm), available at the NBRP B. subtilis, Japan
• GHB6 (ktrC::spec), PubMed (available in Erhard Bremer's and Jörg Stülke's) labs, available at BGSC as 1A955
• GP2264 (ΔktrC::aphA3), available in Jörg Stülke's lab PubMed
• GP2048 (ΔktrC::cat), available in Jörg Stülke's lab PubMed
• GP2079 (ΔktrC::tet), available in Jörg Stülke's lab PubMed
• GP2771 (ΔktrC::spec), available in Jörg Stülke's lab
• GP2083 (ktrA-ktrB::aphA3 ktrC::tet), available in Jörg Stülke's lab PubMed
• BKE14510 (ktrC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTGGTGCTCCTTTATA, downstream forward: _UP4_TAGCAGCCAAATAAGCCGTC
• BKK14510 (ktrC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTGGTGCTCCTTTATA, downstream forward: _UP4_TAGCAGCCAAATAAGCCGTC

Expression vector

• pGP2994: expression of Strep-KtrC in B. subtilis suitable for SPINE (based on pGP380), available in Jörg Stülke's lab
• pGP2995: expression of KtrC-Strep in B. subtilis suitable for SPINE (based on pGP382), available in Jörg Stülke's lab

Expression vectors

• pGP2907 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

Labs working on this gene/protein

• Erhard Bremer, University of Marburg, Germany Homepage
• Inga Hänelt, Frankfurt, Germany Homepage
• João H Morais-Cabral, University of Porto, Portugal Homepage
• Jörg Stülke, University of Göttingen, Germany Homepage

References

Reviews

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Original publications

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