asnO

asparagine synthase (glutamine-hydrolysing)

Locus: BSU_10790

Isoelectric point: 5.70

Molecular weight: 70.54 kDa

Protein length: 614 aa

Gene length: 1845 bp

Function: biosynthesis of asparagine

Product: asparagine synthase (glutamine-hydrolysing)

Essential: no

E.C.: 6.3.5.4

Synonyms: yisO,yucB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of aspartate/ asparagine
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 1,157,237 1,159,081

Phenotypes of a mutant

• inactivation of asnO renders cells asporogenous PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-aspartate + L-glutamine --> AMP + diphosphate + H⁺ + L-asparagine + L-glutamate (according to UniProt)

Protein family

• asparagine synthetase family (with AsnB and AsnH, according to UniProt)

Domains

• Glutamine amidotransferase type-2 domain (aa 2-216) (according to UniProt)

Structure

• 1CT9 (from E. coli, 27% identity) PubMed

Expression and Regulation

Operons

• Genes: asnO
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulation

  • expressed during sporulation (SigE) PubMed

Biological materials

Mutant

• BKE10790 (asnO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGACATCAGCTCCAAT, downstream forward: _UP4_TAGGTTCAATACAAATGTGA
• BKK10790 (asnO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGACATCAGCTCCAAT, downstream forward: _UP4_TAGGTTCAATACAAATGTGA

References

Reviews

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Original publications

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