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kinE
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kinE

two-component sensor kinase, phosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F], part of the [SW|phosphorelay]
locus
BSU_13530
pI
5.49
mw
85.32 kDa
protein length
738 aa Sequence Blast
gene length
2217 bp Sequence Blast
function
initiation of [SW|sporulation]
product
two-component sensor kinase
essential
no
synonyms
ykrQ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.1|The kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.1|The kinases]

    • Gene

    Coordinates
    1,419,213 1,421,429

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F]
  • ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

    • Paralogous protein(s)

  • [protein|4EE48E4931F662E51586DB6D01E91C688329222D|CssS], [protein|52BB660B0CAB36C74F1D47963235846B45AF78AB|YclK], [protein|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|YvrG], [protein|9E8A6AB3920BFBFDAAD1F2E4F333A32354ABB25B|YkoH]

    • [SW|Domains]

  • two transmembrane segments
  • 4 [SW|PAS domain]s (aa 29-99, aa 150-220, aa 271-342, aa 391-462) (according to UniProt)
  • [SW|Histidine kinase domain] (aa 523-729) (according to UniProt)

    • Modification

  • autophosphorylation on a His residue

    • Structure

  • [PDB|3D36] (Geobacillus stearothermophilus [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB] in complex with the inhibitor [protein|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|Sda], corresponds to aa 510 ... 727, 43% identity) [pubmed|19101565]

    • Expression and Regulation

    Operons

    genes
    [gene|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]-[gene|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt]
    description
    [Pubmed|2506524]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2506524], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • MGNA-B319 (ykrQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1318 NBRP B. subtilis, Japan]
  • BKE13530 ([gene|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCATGTACACTCCTTCT, downstream forward: _UP4_GAATGAACTACTATACGACA
  • BKK13530 ([gene|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCATGTACACTCCTTCT, downstream forward: _UP4_GAATGAACTACTATACGACA

    • References

  • 10094672,11069677,2506524,19101565