oxdD [2018-02-13T06:36:18.000Z]

oxalate decarboxylase, inner spore coat protein

Locus: BSU18670

Isoelectric point: 5.36

Molecular weight: 43.39 kDa

Protein length: 392 aa

Gene length: 1176 bp

Function: protection of the spore

Product: oxalate decarboxylase

Essential: no

E.C.: 4.1.1.2

Synonyms: yoaN

Genomic Context

Loading

Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Class V
• Lifestyles → Coping with stress → Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

Gene

Coordinates: 2,037,601 → 2,038,779

The protein

Catalyzed reaction/ biological activity

• Oxalate = formate + CO₂ (according to Swiss-Prot)

Protein family

• UPF0361 family (according to Swiss-Prot)

Paralogous protein(s)

• OxdC

Structure

• 1L3J (OxdC, 59% identity) PubMed

Localization

• inner spore coat, localization depends on SafA PubMed

Expression and Regulation

Operons

• Genes: oxdD
  Description: PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon

  Regulatory mechanism

  • GerE: repression, PubMed, in GerE regulon

  Regulation

  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

Biological materials

Mutant

• MGNA-A842 (yoaN::erm), available at the NBRP B. subtilis, Japan
• BKE18670 (ΔoxdD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCACCTCGTTTTAGAAA, downstream forward: _UP4_TAAAGACGTAACAGATCACA
• BKK18670 (ΔoxdD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCACCTCGTTTTAGAAA, downstream forward: _UP4_TAAAGACGTAACAGATCACA

References

Reviews

Loading

Original publications

Loading