opuBD

choline and arsenocholine ABC transporter (membrane protein)

Locus: BSU_33700

Isoelectric point: 10.06

Molecular weight: 23.78 kDa

Protein length: 226 aa

Gene length: 681 bp

Function: compatible solute transport

Product: choline and arsenocholine ABC transporter (membrane protein)

Essential: no

Synonyms: proZ

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → Coping with hyper-osmotic stress
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,459,806 3,460,486

The protein

Catalyzed reaction/ biological activity

• uptake of choline and arsenocholine PubMed

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• CysTW subfamily (according to UniProt)

Paralogous protein(s)

• OpuCD

Domains

• ABC transmembrane type-1 domain (aa 22-202) (according to UniProt)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: opuBA-opuBB-opuBC-opuBD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • GbsR: repression, (transcriptional roadblock) PubMed, in GbsR regulon
  • OpcR: repression, (sterical interference with RNA polymerase access) PubMed, in OpcR regulon
  • RemA: activation, PubMed, in RemA regulon

  Regulation

  • induced by choline (GbsR) PubMed
  • expression increases with increasing salinity PubMed

  Additional information

  • S1290 acts as antisense RNA for the opuBA-opuBB-opuBC-opuBD operon PubMed
  • expression starts with a delay after osmotic upshift due to transcriptional interference of RNA polymerase complexes driving synthesis of the converging opuB and S1290 mRNAs PubMed

Biological materials

Mutant

• BKE33700 (opuBD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTTCATTGACCTCTTCC, downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG
• BKK33700 (opuBD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTTCATTGACCTCTTCC, downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG

References

Reviews

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Original publications

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