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radA [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]
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radA [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

6-O-methylguanine-DNA methyltransferase, negative effector of DisA activity, participates in the stabilization and/or processing of holliday junction intermediates
locus
BSU00870
pI
8.00
mw
49.00 kDa
function
homologous recombination , control of c-di-AMP formation
product
6-O-methylguanine-DNA methyltransferase
essential
no
synonyms
sms, yacJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|metabolism of signalling nucleotides], [SW|DNA repair/ recombination], [SW|general stress proteins (controlled by SigB)], [SW|heat shock proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CtsR regulon], [SW|SigB regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    106,096 -> 107,472

    The protein

    Protein family

  • RadA subfamily (according to Swiss-Prot)

    • [SW|Interactions]

  • [protein|search|RadA]-[protein|search|DisA] [Pubmed|23760274]

    • Expression and Regulation

    Operon

  • ''[protein|search|ctsR]-[protein|search|mcsA]-[protein|search|mcsB]-[protein|search|clpC]-[protein|search|radA]-[protein|search|disA]'' [Pubmed|8793870]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8793870], [protein|search|SigB] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2]

    • Regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|11544224], induced by heat ([protein|search|CtsR]) [Pubmed|9987115]

    • Regulatory mechanism

  • [protein|search|CtsR]: transcription repression [Pubmed|9987115]

    • Biological materials

    Mutant

  • BKG1 (''[protein|search|radA]''::''spc''), available in [SW|Jörg Stülke]'s lab
  • BKG2 (''[protein|search|radA]-[protein|search|disA]::spc''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP2693: expression in ''E. coli'', with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

    • References

  • 8793870,9987115,15317759,23760274,11544224,25484163,11810266