ptsI

Enzyme I, general (non sugar-specific) component of the PTS

Locus: BSU_13910

Isoelectric point: 4.59

Molecular weight: 62.90 kDa

Protein length: 570 aa

Gene length: 1713 bp

Function: PTS-dependent sugar transport

Product: phosphotransferase system (PTS) enzyme I

Essential: no

E.C.: 2.7.3.9

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Phosphotransferase system → General PTS proteins
• Information processing → Genetics → Newly identified competence genes
• Groups of genes → Phosphoproteins → Phosphorylation on a His residue
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 1,459,650 1,461,362

Phenotypes of a mutant

• poorly transformable PubMed

The protein

Catalyzed reaction/ biological activity

• L-histidyl-[protein] + phosphoenolpyruvate --> N^π-phospho-L-histidyl-[protein] + pyruvate (according to UniProt)
• PEP-dependent autophosphorylation on His-189, transfer of the phosphoryl group to HPr]] (His-15)

Protein family

• PEP-utilizing enzyme family (according to UniProt)

Domains

• HPr binding site (N-Terminal Domain)
• pyruvate binding site (C-Terminal Domain)
• pyrophosphate/phosphate carrier histidine (central Domain)

Modification

• transient autophosphorylation on His-189
• in vivo also phosphorylated on Ser-34 or Ser-36 PubMed

Cofactors

• Magnesium

Structure

• 2WQD (Enzyme I from Staphylococcus aureus, 68% identity) PubMed

Localization

• cytoplasm, even distribution PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: ptsH-ptsI
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

• Genes: ptsG-ptsH-ptsI
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • GlcT: antitermination, via the GlcT-dependent RNA switch PubMed, in GlcT regulon

  Regulation

  • expression activated by glucose (2 fold) (GlcT) PubMed

Biological materials

Mutant

• available in Jörg Stülke's lab:
• GP864 (ΔptsI::ermC)
• GP778 (ΔglcT-ptsG-ptsH-ptsI::spc) PubMed
• BKE13910 (ptsI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACCAATCCCTTTTAATTCTT, downstream forward: _UP4_TAATGTACAAAAACCAGACG
• BKK13910 (ptsI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACCAATCCCTTTTAATTCTT, downstream forward: _UP4_TAATGTACAAAAACCAGACG

GFP fusion

• GP1268, ptsI-cfp, available in Jörg Stülke's lab PubMed

Expression vectors

• pAG3 (His-tag) PubMed, available in Galinier lab
• for expression, purification in E. coli (His-tag), in pWH844: pGP813 available in Jörg Stülke's lab

Labs working on this gene/protein

• Jörg Stülke, University of Göttingen, Germany Homepage

References

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