ptsI

Enzyme I, general (non sugar-specific) component of the PTS

locus: BSU_13910

pI: 4.59

mw: 62.90 kDa

protein length: 570 aa

gene length: 1713 bp

function: PTS-dependent sugar transport

product: phosphotransferase system (PTS) enzyme I

essential: no

ec: 2.7.3.9

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.1|General PTS proteins]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 1,459,650 1,461,362

Phenotypes of a mutant

• poorly transformable [pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• L-histidyl-[protein] + phosphoenolpyruvate --> N^π-phospho-L-histidyl-[protein] + pyruvate (according to UniProt)
• PEP-dependent autophosphorylation on His-189, transfer of the phosphoryl group to [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr]]] (His-15)

Protein family

• [SW|PEP-utilizing enzyme family] (according to UniProt)

[SW|Domains]

• HPr binding site (N-Terminal Domain)
• pyruvate binding site (C-Terminal Domain)
• pyrophosphate/phosphate carrier histidine (central Domain)

Modification

• transient autophosphorylation on His-189
• ''in vivo'' also phosphorylated on Ser-34 or Ser-36 [Pubmed|17218307]

[SW|Cofactors]

• Magnesium

Structure

• [PDB|2WQD] (Enzyme I from ''Staphylococcus aureus'', 68% identity) [Pubmed|19801641]

[SW|Localization]

• cytoplasm, even distribution [Pubmed|23475962]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|29B793660E4D30C0656248F3EF403FEF76FB9025|ptsH]-[gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]
  description: [Pubmed|11902727]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11902727], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

• genes: [gene|B5E7EB475434E96786C577AE709A21BD702733D8|ptsG]-[gene|29B793660E4D30C0656248F3EF403FEF76FB9025|ptsH]-[gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]
  description: [Pubmed|11902727]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11902727], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • [protein|BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449|GlcT]: antitermination, via the [protein|BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449|GlcT]-dependent [SW|RNA switch] [PubMed|9765562], in [regulon|BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449|GlcT regulon]

  regulation

  • expression activated by glucose (2 fold) ([protein|search|GlcT]) [Pubmed|12850135]

Biological materials

Mutant

• available in [SW|Jörg Stülke]'s lab:
• GP864 (Δ[gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]::ermC)
• GP778 (Δ[gene|BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449|glcT]-[gene|B5E7EB475434E96786C577AE709A21BD702733D8|ptsG]-[gene|29B793660E4D30C0656248F3EF403FEF76FB9025|ptsH]-[gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]::spc) [Pubmed|22722928]
• BKE13910 ([gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCAATCCCTTTTAATTCTT, downstream forward: _UP4_TAATGTACAAAAACCAGACG
• BKK13910 ([gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCAATCCCTTTTAATTCTT, downstream forward: _UP4_TAATGTACAAAAACCAGACG

GFP fusion

• GP1268, [gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]-cfp, available in [SW|Jörg Stülke]'s lab [pubmed|23475962]

Expression vectors

• pAG3 (His-tag) [Pubmed|9237995], available in [SW|Galinier] lab
• for expression, purification in ''E. coli'' (His-tag), in [SW|pWH844]: pGP813 available in [SW|Jörg Stülke]'s lab

labs

• [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

References

• 12850135,17218307,9721285,20081037,19801641,23475962,24129255,15378759,28189581