spoIIM

required for dissolution of the septal cell wall

locus: BSU_23530

pI: 9.84

mw: 24.15 kDa

protein length: 214 aa

gene length: 645 bp

function: dissolution of the septal cell wall

product: sporulation protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 2,450,403 2,451,047

The protein

[SW|Localization]

• cytoplasm (according to Swiss-Prot)
• the complex of [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|SpoIID], [protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|SpoIIM], and [protein|C9817A54C8E72193280E393D7BD3375BD1751738|SpoIIP] localizes at the leading edge of the mother cell engulfing membrane and is essential and rate limiting for membrane migration [Pubmed|20382772,12502745]

Expression and Regulation

Operons

• genes: [gene|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]
  description: [Pubmed|8501065]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|8501065], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|8501065]

Biological materials

Mutant

• MGNA-C416 (spoIIM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2414 NBRP B. subtilis, Japan]
• BKE23530 ([gene|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCGTTTCCTCCCGCCC, downstream forward: _UP4_TAAAATCATTATTAAAAGAA
• BKK23530 ([gene|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCGTTTCCTCCCGCCC, downstream forward: _UP4_TAAAATCATTATTAAAAGAA

References

Reviews

• 32660383

Original publications

• 17376078,11886548,15752199,8501065,15383836,23859254,20382772,12502745,31282858