ansR [Mon Aug 31 2015 13:55:26 GMT+0200 (CEST)]

transcriptional repressor (Xre family) of the ansA-ansB operon

locus: BSU23590

pI: 4.81

mw: 13.00 kDa

function: negative regulation of the ansA-ansB operon

product: transcriptional regulator (Xre family)

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.6|Utilization of asparagine/ aspartate]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

[SW|Categories] containing this gene/protein

• [SW|utilization of amino acids], [SW|transcription factors and their control]

This gene is a member of the following [SW|regulons]

• [SW|AnsR regulon]

Gene

Phenotypes of a mutant

• the ''ansR'' mutation allows glutamate utilization of a ''[protein|search|rocG] [protein|search|gudB]'' mutant [Pubmed|21219666]

The protein

Protein family

• [SW|Xre family]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• ''ansR'' [Pubmed|8478318]

Regulation

• induced in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]

Regulatory mechanism

• [protein|search|AnsR]: negative autoregulation [Pubmed|11914346]

Biological materials

Mutant

• GP1152 (del tet) available in the [SW|Stülke] lab

Expression vector

• expression of native ''ansR'' in ''B. subtilis'': pGP873 (in [SW|pBQ200]), available in [SW|Stülke] lab

References

• 8478318,11914346,21219666