lrpB

transcriptional regulator ([SW|Lrp family]) involved in repression of [gene|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA] transcription and [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB]-dependent [SW|sporulation]

locus: BSU_05060

pI: 6.68

mw: 17.18 kDa

protein length: 149 aa

gene length: 450 bp

function: repression of [gene|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA] transcription and [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB]-dependent [SW|sporulation]

product: transcription regulator ([SW|Lrp family])

essential: no

synonyms: yddP

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay]

Gene

Coordinates: 552,052 552,501

The protein

Protein family

• ([SW|Lrp family])

[SW|Domains]

• [SW|HTH asnC-type domain] (aa 3-64) (according to UniProt)

Structure

• [PDB|2CFX] ([protein|AC2618C53DA020F2CD5192AEE462D15E8EE0F57F|LrpC], 31% identity) [pubmed|16528101]

Biological materials

Mutant

• MGNA-C110 (yczA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2108 NBRP B. subtilis, Japan]
• BKE05060 ([gene|1437D66EABB5D7FF4F8B9EBAD624510D86F0C8B4|lrpB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTAACTCCTTTCACAG, downstream forward: _UP4_TAATGCGCTTGTTATTATAA
• BKK05060 ([gene|1437D66EABB5D7FF4F8B9EBAD624510D86F0C8B4|lrpB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTAACTCCTTTCACAG, downstream forward: _UP4_TAATGCGCTTGTTATTATAA

References

Research papers

• 16528101