ywjH [2019-02-19 18:23:48]

transaldolase

locus: BSU37110

pI: 5.88

mw: 22.77 kDa

protein length: 212 aa

gene length: 639 bp

function: pentose phosphate pathway

product: transaldolase

essential: no

ec: 2.2.1.2

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.3|Pentose phosphate pathway]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 3,807,754 3,808,392

The protein

Catalyzed reaction/ biological activity

• Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate (according to Swiss-Prot)

Protein family

• Type 3B subfamily (according to Swiss-Prot)

Modification

• phosphorylation on Ser-39 [Pubmed|17218307,16493705,17726680], ''in vitro'' phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC] [Pubmed|20389117]

Structure

• [PDB|3R8R] [Pubmed|22212631]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]-[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]
  description: [Pubmed|11489127]

  regulation

  • constitutively expressed [Pubmed|11489127]

Biological materials

Mutant

• MGNA-B264 (ywjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1263 NBRP B. subtilis, Japan]
• BKE37110 ([gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCAAAAAGCCTCCCT, downstream forward: _UP4_TAATGAAAGGGGCGGCAAAC
• BKK37110 ([gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCAAAAAGCCTCCCT, downstream forward: _UP4_TAATGAAAGGGGCGGCAAAC

Expression vectors

• for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP819, available in [SW|Stlke] lab, [pubmed|20389117]
• pGP1790 (expression of ''[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]'' in ''B. subtilis'', in [SW|pGP1389]), available in [SW|Stlke] lab
• GP1409 (''ywjH''-''Strep'' ''(spc)'') & GP1411 (''ywjH''-''Strep'' ''(cat)''), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Stlke] lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stlke] lab

FLAG-tag construct

• GP1407 (spc, based on [SW|pGP1331]), available in the [SW|Stlke] lab

References

Reviews

• 25267444

Original publications

• 17726680,17218307,16493705,11489127,20389117,21857661,22212631,15378759