ywjH

transaldolase

Locus: BSU_37110

Isoelectric point: 5.88

Molecular weight: 22.77 kDa

Protein length: 212 aa

Gene length: 639 bp

Function: pentose phosphate pathway

Product: transaldolase

Essential: no

E.C.: 2.2.1.2

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → Pentose phosphate pathway
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 3,807,754 3,808,392

The protein

Catalyzed reaction/ biological activity

• D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate --> β-D-fructose 6-phosphate + D-erythrose 4-phosphate (according to UniProt)

Protein family

• transaldolase family (single member, according to UniProt)

Modification

• phosphorylation on Ser-39 PubMed, in vitro phosphorylated by PrkC PubMed

Structure

• 3R8R PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: fbaA-ywjH
  Description: PubMed

  Regulation

  • constitutively expressed PubMed

Biological materials

Mutant

• MGNA-B264 (ywjH::erm), available at the NBRP B. subtilis, Japan
• BKE37110 (ywjH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCAAAAAGCCTCCCT, downstream forward: _UP4_TAATGAAAGGGGCGGCAAAC
• BKK37110 (ywjH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCAAAAAGCCTCCCT, downstream forward: _UP4_TAATGAAAGGGGCGGCAAAC

Expression vectors

• for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP819, available in Jörg Stülke's lab, PubMed
• pGP1790 (expression of ywjH in B. subtilis, in pGP1389), available in Jörg Stülke's lab
• GP1409 (ywjH-Strep (spc)) & GP1411 (ywjH-Strep (cat)), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1407 (spc, based on pGP1331), available in Jörg Stülke's lab

References

Reviews

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Original publications

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