yloM [2019-08-01 11:07:50]

similar to RNA-binding Sun protein, similar to S-adenosyl-L-methionine-dependent 16S rRNA m5C967 methyltransferase

Locus: BSU_15740

Isoelectric point: 6.95

Molecular weight: 50.11 kDa

Protein length: 447 aa

Gene length: 1344 bp

Function: rRNA modification

Product: S-adenosyl-L-methionine-dependent 16S rRNA m5C967 methyltransferase

Essential: no

Synonyms: sun

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → rRNA modification and maturation/ based on similarity

Gene

Coordinates: 1,647,939 1,649,282

The protein

Catalyzed reaction/ biological activity

• S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing C(5)-methylcytosine (according to Swiss-Prot)

Protein family

• Methyltransferase superfamily (according to UniProt)
• class I-like SAM-binding methyltransferase superfamily (according to UniProt)

Structure

• 1SQF (E. coli Fmu protein, complex with SAM) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: defA-fmt-yloM-yloN
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

• Genes: yloC-remA-gmk-yloH-yloI-priA-defA-fmt-yloM-yloN

  Description

  • PubMed

Biological materials

Mutant

• MGNA-B373 (yloM::erm), available at the NBRP B. subtilis, Japan
• BKE15740 (yloM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGCCTCAAGGGCGATGTCAC, downstream forward: _UP4_AGCATGAGAAAGAAGGGATA
• BKK15740 (yloM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGCCTCAAGGGCGATGTCAC, downstream forward: _UP4_AGCATGAGAAAGAAGGGATA

References

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