dhbB

isochorismatase

Locus: BSU_31970

Isoelectric point: 4.43

Molecular weight: 34.95 kDa

Protein length: 312 aa

Gene length: 939 bp

Function: biosynthesis of the siderophore bacillibactin

Product: isochorismatase

Essential: no

E.C.: 3.3.2.1

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Homeostasis → Acquisition of iron → Acquisition of iron / Other
• Metabolism → Additional metabolic pathways → Iron metabolism → Acquisition of iron / Other
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Other proteins required for biofilm formation

Gene

Coordinates: 3,287,675 3,288,613

Phenotypes of a mutant

• defective in biofilm formation, this can be suppressed by the addition of 2,3-dihydroxybenzoate to the medium PubMed

The protein

Catalyzed reaction/ biological activity

• Isochorismate + H₂O --> 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate (according to UniProt)

Protein family

• Isochorismatase family (according to UniProt)

Domains

• Carrier domain (aa 229-302) (according to UniProt)

Structure

• 2FQ1 (from E. coli, 49% identity) PubMed

Expression and Regulation

Operons

• Genes: dhbA-dhbC-dhbE-dhbB-dhbF-ybdZ
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigI: sigma factor, PubMed, in SigI regulon

  Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • Kre: repression, in Kre regulon

  Regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed
  • expression of the operon is strongly induced at the beginning of biofilm formation PubMed

  Additional information

  • the dhbA-dhbC-dhbE-dhbB-dhbF operon is strongly unregulated in a kre mutant PubMed

Biological materials

Mutant

• BKE31970 (dhbB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGAACACTTCCTCTCA, downstream forward: _UP4_TTATAAAAGGAGGGTAAACA
• BKK31970 (dhbB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGAACACTTCCTCTCA, downstream forward: _UP4_TTATAAAAGGAGGGTAAACA

References

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