citT [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

two-component response regulator, regulation of citrate uptake

locus: BSU07590

pI: 6.00

mw: 25.00 kDa

function: regulation of citrate uptake

product: two-component response regulator

essential: no

synonyms: yflQ

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources], [SW|transcription factors and their control], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence): 832,545 -> 833,225

Phenotypes of a mutant

• no growth with citrate as sole carbon source [Pubmed|10972810]

The protein

Catalyzed reaction/ biological activity

• binding to the promoter of the ''[protein|search|citM]-[protein|search|yflN]'' operon and activation of transcription in the presence of citrate [Pubmed|10972810]

Modification

• phosphorylated by [protein|search|CitS] on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

[SW|Interactions]

• [protein|search|CitS]-[protein|search|CitT]

Expression and Regulation

Operon

• ''[protein|search|citS]-[protein|search|citT]-[protein|search|yflP]'' [Pubmed|10972810]

Biological materials

Mutant

• MGNA-C250 (citT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2248 NBRP B. subtilis, Japan]

References

• 10094672,16842348,10972810