prfB [Mon Jan 18 2016 18:49:38 GMT+0100 (CET)]

peptide chain release factor 2

locus: BSU35290

pI: 4.00

mw: 41.00 kDa

function: translation

product: peptide chain release factor 2

essential: yes

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|translation], [SW|essential genes]

Gene

Coordinates on the chromosome (coding sequence): 3,627,139 -> 3,628,240

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfB is highly specific for a U in the first stop-codon position and recognizes UAA and UGA

Protein family

• prokaryotic/mitochondrial release factor family (according to Swiss-Prot)

Structure

• [PDB|1MI6] (from ''E. coli'', 45% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• ''[protein|search|secA]-[protein|search|prfB]'' [Pubmed|9882663]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|9882663]

References

• 9882663,20512119,25217634