prfB

peptide chain release factor 2

Locus: BSU_35290

Isoelectric point: 4.85

Molecular weight: 41.91 kDa

Protein length: 366 aa

Gene length: 1102 bp

Function: translation

Product: peptide chain release factor 2

Essential: yes

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Translation factors
• Groups of genes → Essential genes

Gene

Coordinates: 3,627,139 3,628,240

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfB is highly specific for a U in the first stop-codon position and recognizes UAA and UGA

Protein family

• prokaryotic/mitochondrial release factor family (with PrfA, according to UniProt)

Structure

• 1MI6 (from E. coli, 45% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: secA-prfB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

References

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