bdbB

thiol-disulfide oxidoreductase

Locus: BSU_21440

Isoelectric point: 9.41

Molecular weight: 17.00 kDa

Protein length: 148 aa

Gene length: 447 bp

Function: oxidative folding of proteins

Product: thiol-disulfide oxidoreductase

Essential: no

Synonyms: yolK

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Chaperones/ protein folding
• Prophages and mobile genetic elements → Prophages → SP-beta prophage
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,265,225 2,265,671

The protein

Catalyzed reaction/ biological activity

• oxidative folding of SunA (together with BdbC)

Protein family

• DsbB family (with BdbC, according to UniProt)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: sunA-sunT-bdbA-yolJ-bdbB
  Description: PubMed

  Regulatory mechanism

  • Rok: repression, PubMed, in Rok regulon
  • Abh: activation, PubMed, in Abh regulon
  • DnaA: repression, Rok-DnaA PubMed, in DnaA regulon
  • YvrHb: activation, PubMed, in YvrHb regulon
  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • repressed by Rok-DnaA PubMed
  • expression starts in the stationary phase PubMed
  • expression is heterogeneous in the population, this is mediated by AbrB, Rok, and Spo0A PubMed

  Additional information

  • the mRNA is very stable (> 15 min) PubMed
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

Biological materials

Mutant

• BKE21440 (bdbB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAATGATTTTACATATCTTG, downstream forward: _UP4_TGAATAAGAAGTAACCCGCC
• BKK21440 (bdbB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAATGATTTTACATATCTTG, downstream forward: _UP4_TGAATAAGAAGTAACCCGCC

References

Reviews

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Original publications

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