yvrA

ABC transporter (ATP binding domain) for cobalamin

Locus: BSU_33160

Isoelectric point: 5.01

Molecular weight: 48.47 kDa

Protein length: 442 aa

Gene length: 1329 bp

Function: uptake of cobalamin

Product: ABC transporter (ATP binding domain)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of cofactors
• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of cobalamine (B12))
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,401,141 3,402,469

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 1-238) (according to UniProt)

Structure

• 4G1U (from Yersinia pestis, N-terminal domain, aa 1 .. 255, 41% identity) PubMed

Expression and Regulation

Operons

• Genes: yvrC-yvrB-yvrA-yvqK

  Regulatory mechanism

  • B12 riboswitch: termination, upon binding of B12 PubMed, in B12 riboswitch

  Regulation

  • expression is decreased in the presence of cobalamin (vitamin B12) (B12 riboswitch) PubMed

Biological materials

Mutant

• MGNA-B043 (yvrA::erm), available at the NBRP B. subtilis, Japan
• BKE33160 (yvrA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCCCGACAGCCCTTCTGCCT, downstream forward: _UP4_TAAGTCTGTAAAGGAGATTC
• BKK33160 (yvrA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCCCGACAGCCCTTCTGCCT, downstream forward: _UP4_TAAGTCTGTAAAGGAGATTC

References

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