DNA repair polymerase/ ligase in non-homologous end joining DNA repair
function
non-homologous end joining DNA repair, repair of gapped DNA substrates
product
DNA repair polymerase/ ligase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.3|Spore-encoded non-homologous end joining system][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other][SW|Categories] containing this gene/protein
[SW|DNA repair/ recombination], [SW|sporulation proteins]This gene is a member of the following [SW|regulons]
[SW|SigG regulon], [SW|SpoVT regulon]Gene
Coordinates on the chromosome (coding sequence)
1,404,518 -> 1,406,353
Phenotypes of a mutant
sensitivity to ionizing radiation in the stationary phase [Pubmed|12215643]sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat [Pubmed|16497325,17293412]a ''[protein|search|ykoV]-[protein|search|ligD]'' double mutant is sensitive to radiation [Pubmed|24123749]The protein
Catalyzed reaction/ biological activity
has inherent polymerization and ligase activities that allow it to fill the short gaps that can arise after realignment of the broken ends and to seal the resulting nicks, contributing to genome stability during the stationary phase and germinationhas an intrinsic 5'-2-deoxyribose-5-phosphate (dRP) lyase activity located at the N-terminal ligase domain [Pubmed|26826709][SW|Domains]
N-terminal DNA ligase catalytic domain (aa 1 - 331) linked to a C-terminal polymerase domain (aa 332 - 611) [Pubmed|23691176][SW|Interactions]
[protein|search|YkoV]-[protein|search|LigD], to stimulate [SW|LigD] activity [Pubmed|23691176,26961308]Expression and Regulation
Operon
''[protein|search|ykoV]-[protein|search|ligD]'' [Pubmed|16497325][SW|Sigma factor]
[protein|search|SigG] [Pubmed|16497325]Regulation
expressed during [SW|sporulation] in the forespore ([protein|search|SigG], [SW|SpoVT]) [Pubmed|16497325]Regulatory mechanism
[SW|SpoVT]: transcription activation [Pubmed|16497325]Biological materials
Mutant
BP141 (''[protein|search|ykoV]-[protein|search|ligD]''::''kan'') available in [SW|Fabian Commichau]'s labBP142 (''[protein|search|ykoW]-[protein|search|ykoV]-[protein|search|ligD]''::''kan'') available in [SW|Fabian Commichau]'s labReferences
Reviews
Original publications
16497325,23691176,12215643,11075926,11566200,17293412,24123749,24123749,25355514,26826709,26961308 
