murR

transcriptional repressor of genes required for the utilization of N-acetylmuramic acid

locus: BSU_01690

pI: 6.87

mw: 30.56 kDa

protein length: 283 aa

gene length: 852 bp

function: regulation of muramic acid utilization

product: transcriptional repressor

essential: no

synonyms: ybbH

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.3|Utilization of cell wall components]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.17|Utilization of amino sugars]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.3|Utilization of amino sugars]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 191,183 192,034

The protein

[SW|Domains]

• HTH rpiR-type domain (aa 3-79) (according to UniProt)
• [SW|SIS domain] (aa 123-264) (according to UniProt)

Structure

• [PDB|2O3F] (N-terminal domain)

Expression and Regulation

Operons

• genes: [gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]-[gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]-[gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]-[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]-[gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]-[gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|ybbC]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR]: repression, [pubmed|30038046], in [regulon|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR regulon]

  regulation

  • expressed in late exponential and early stationary phase [Pubmed|20400549]

Biological materials

Mutant

• MGNA-B951 (ybbH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1950 NBRP B. subtilis, Japan]
• BKE01690 ([gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTGGCCATTCTAGTTCTCT, downstream forward: _UP4_TAAGGCATCTTGAAGGGGAG
• BKK01690 ([gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTGGCCATTCTAGTTCTCT, downstream forward: _UP4_TAAGGCATCTTGAAGGGGAG

References

• 15060041,20400549,10627040,22383849,30038046