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spoIIIE [2019-09-04 09:16:59]
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spoIIIE [2019-09-04 09:16:59]

ATP-dependent dsDNA translocase, resolution of chromosomal dimers after DNA replication, transports the forespore chromosome across the sporulation septum
Locus
BSU_16800
Isoelectric point
6.76
Molecular weight
86.96 kDa
Protein length
789 aa Sequence Blast
Gene length
2370 bp Sequence Blast
Function
chromosome partition during sporulation
Product
ATP-dependent dsDNA translocase required for chromosome partition
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → DNA condensation/ segregation
  • Lifestyles → Sporulation → Sporulation/ other
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,752,272 1,754,641

    The protein

    Catalyzed reaction/ biological activity

  • transports the forespore chromosome across the sporulation septum PubMed
  • translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes PubMed
  • the two DNA translocases SftA and SpoIIIE synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively PubMed
  • binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG PubMed

    • Protein family

  • FtsK/SpoIIIE/SftA family (with SftA, according to UniProt)

    • Paralogous protein(s)

  • SftA

    • Domains

  • N-terminal transmembrane domain (4 helices) PubMed
  • 134 aa linker PubMed
  • 512 aa motor domain at the C-terminus PubMed

    • Structure

  • 2IUT (FtsK from Pseudomonas aeruginosa, partial, 48% identity, complex with SftA) PubMed

    • Localization

  • free diffusion in the membrane, no enrichment at septa PubMed
  • transmembrane domain mediates dynamic localization to active division sites PubMed
  • complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of sporulation and division septa PubMed

    • Expression and Regulation

    Operons

    Genes
    spoIIIE-ymfC
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
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    Biological materials

    Mutant

  • BKE16800 (spoIIIE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACACTCATCACCTTACTGC, downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT
  • BKK16800 (spoIIIE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACACTCATCACCTTACTGC, downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT

    • References

    Reviews

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    Original publications

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