ATP-dependent dsDNA translocase, resolution of chromosomal dimers after DNA replication, transports the forespore chromosome across the sporulation septum
function
chromosome partition during sporulation
product
ATP-dependent dsDNA translocase required for chromosome partition
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][SW|Categories] containing this gene/protein
[SW|DNA condensation/ segregation], [SW|sporulation/ other], [SW|membrane proteins]Gene
Coordinates on the chromosome (coding sequence)
1,752,272 -> 1,754,641
The protein
Catalyzed reaction/ biological activity
transports the forespore chromosome across the sporulation septum [Pubmed|20516200]translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes [Pubmed|19818024]the two DNA translocases [protein|search|SftA] and [SW|SpoIIIE] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively [Pubmed|21239579]binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG [Pubmed|18391964]Paralogous protein(s)
[protein|search|SftA][SW|Domains]
N-terminal transmembrane domain (4 helices) [Pubmed|24297254]134 aa linker [Pubmed|24297254]512 aa motor domain at the C-terminus [Pubmed|24297254]Structure
[PDB|2IUT] (FtsK from ''Pseudomonas aeruginosa'', partial, 48% identity, complex with [SW|SftA]) [Pubmed|16916635][SW|Localization]
transmembrane domain mediates dynamic localization to active division sites [Pubmed|20516200]complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of [SW|sporulation] and division septa [Pubmed|23667326][SW|Interactions]
assembles into ∼45 nm complexes (homo-hexamers) [Pubmed|23667326]Expression and Regulation
Operon
''spoIIIE'' [Pubmed|3129532][SW|Sigma factor]
[protein|search|SigA] [Pubmed|2507870]Biological materials
Mutant
BKE16800 (''spoIIIE''::''erm trpC2'') is available from the [http://bgsc.org Bacillus Genetic Stock Center]References
Reviews
22047950,22934648,23400100,25460803 Original publications
18160039,18593879,3129532,2507870,18593879,23559069,9150232,2492502,11062134,9155041,14680637,7797072,7567988,2507870,8160014,9515703,19818024,19788545,20298190,20516200,21239579,18391964,23667326,11134515,24297254,24769697,25950186,26452092,16916635,26849443,27886417 
