argF

ornithine carbamoyltransferase

Locus: BSU_11250

Isoelectric point: 4.97

Molecular weight: 34.51 kDa

Protein length: 319 aa

Gene length: 960 bp

Function: biosynthesis of arginine

Product: ornithine carbamoyltransferase

Essential: no

E.C.: 2.1.3.3

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of arginine

Gene

Coordinates: 1,203,461 1,204,420

The protein

Catalyzed reaction/ biological activity

• carbamoyl phosphate + L-ornithine --> H⁺ + L-citrulline + phosphate (according to UniProt)

Protein family

• ATCase/OTCase family (with PyrB, according to UniProt)

Structure

• 2EF0 (from Thermus thermophilus hb8, 48% identity, 63% similarity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: argC-argJ-argB-argD-carA-carB-argF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • AhrC: repression, PubMed, in AhrC regulon
  • CodY: repression, PubMed, in CodY regulon
  • YlxR: regulation, PubMed, in YlxR regulon

  Regulation

  • repressed by arginine (AhrC) PubMed
  • induced in the absence of YlxR PubMed

Biological materials

Mutant

• 1A605 ( argF::erm), PubMed, available at BGSC
• 1A606 ( argF::erm), PubMed, available at BGSC
• BKE11250 (argF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTTCCGTATAAACTGGTTT, downstream forward: _UP4_TGAGCCAAACTCAGCAGTTT
• BKK11250 (argF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTTCCGTATAAACTGGTTT, downstream forward: _UP4_TGAGCCAAACTCAGCAGTTT

Expression vector

• pGP2841: IPTG inducible expression, purification in E. coli with C-terminal Strep-tag, in pGP574, available in Jörg Stülke's lab

References

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