rplGB

highly expressed protein, similar to ribosomal protein L7AE family, associated with the ribosome during exponential growth, binds K-turns in RNA switches

Locus: BSU_01090

Isoelectric point: 9.51

Molecular weight: 8.33 kDa

Protein length: 82 aa

Gene length: 249 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ybaB,rpmXB,ktuS

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Ribosomal proteins

Gene

Coordinates: 129,340 129,588

The protein

Catalyzed reaction/ biological activity

• binds K-turns in RNA switches as they occur in the L-box, the S-box and the T-box PubMed

Protein family

• Eukaryotic ribosomal protein eL8 family (with RplGA, according to UniProt)

Structure

• 3V7E (RplGB bound to the [S-box|search|SAM-I riboswitch] aptamer) PubMed

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: rplGB-rpsL-rpsG-fusA-tufA
  Description: PubMed

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
  • the mRNA is processed between rplGB and rpsL by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

Biological materials

Mutant

• BKE01090 (rplGB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAACATATCCTCCAAAG, downstream forward: _UP4_TAACGTACTTTTGTTTTTGC
• BKK01090 (rplGB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAACATATCCTCCAAAG, downstream forward: _UP4_TAACGTACTTTTGTTTTTGC

References

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