patB

cystathione-beta-lyase

locus: BSU_31440

pI: 5.44

mw: 42.63 kDa

protein length: 387 aa

gene length: 1164 bp

function: biosynthesis of methionine

product: cystathione-beta-lyase

essential: no

ec: 4.4.1.8

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]

Gene

Coordinates: 3,228,778 3,229,941

The protein

Catalyzed reaction/ biological activity

• H₂O + L,L-cystathionine --> L-homocysteine + NH₄⁺ + pyruvate (according to UniProt)

Protein family

• [SW|class-II pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[SW|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|3T32] (from ''B. anthracis'', 46% identity, 75% similarity)

Expression and Regulation

Operons

• genes: [gene|11B54D438CDD2EAA4A87A6B99851A3B123EA4A1F|patB]
  description: [Pubmed|9274030]

Biological materials

Mutant

• 1A952 ( ''patB''::''cat''), [Pubmed|15760717], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A952&Search=1A952 BGSC]
• BKE31440 ([gene|11B54D438CDD2EAA4A87A6B99851A3B123EA4A1F|patB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGTTACCCCCTCATG, downstream forward: _UP4_TAAATATAGCTGTAAACGCC
• BKK31440 ([gene|11B54D438CDD2EAA4A87A6B99851A3B123EA4A1F|patB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGTTACCCCCTCATG, downstream forward: _UP4_TAAATATAGCTGTAAACGCC

References

• 9274030,15760717