floA [2017-8-11 16:0:3]

flottilin-like protein (in addition to FloT), resistence protein (against sublancin), accessory role in resistance to cefuroxime

locus: BSU25380

pI: 4.92

mw: 35.48 kDa

protein length: 331 aa

gene length: 993 bp

function: resistance against sublancin

product: flottilin-like protein

essential: no

synonyms: yqfA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.7|Membrane dynamics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,617,449 → 2,618,444

Phenotypes of a mutant

• reduced [SW|protein secretion] [Pubmed|23651456]
• a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant does not induce [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|KinC]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]
• a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]
• a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant has a [SW|sporulation] defect, due to the lack of [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|FtsH] [Pubmed|22882210]
• a ''[gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' mutant produces increased amount of pulcherriminic acid [Pubmed|25909364]
• a ''[gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' mutant has reduced sensitivity to vancomycin [Pubmed|25909364]

The protein

Catalyzed reaction/ biological activity

• controls protease activity of [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|FtsH] [Pubmed|24222488]

Protein family

• UPF0365 family (according to Swiss-Prot)

[SW|Localization]

• membrane [Pubmed|18763711]
• co-localizes with [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|FloT] in discrete foci in the membrane [Pubmed|20713508]
• forms focal structure in the cytoplasma membrane [Pubmed|22753055]
• 13 foci [protein|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|FloA] of are detected in strain 3610 [Pubmed|25909364]

Expression and Regulation

Operons

• genes: [gene|F6B0D27B434D81B7A680555E4457F266B6206B7D|yqeZ]-[gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]-[gene|A80317C3B8DF06E2187E02A05C13845508C18B40|yqfB]
  description: [Pubmed|11866510]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|11866510], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

  regulation

  • expressed under stress conditions ([protein|search|SigW]) [Pubmed|11866510]

Biological materials

Mutant

• MGNA-C443 (yqfA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2441 NBRP B. subtilis, Japan]
• JS119 (3610 markerless), available in [SW|Daniel Lopez]'s lab
• DL1401 (168 ''ΔfloA''::mls), available in [SW|Daniel Lopez]'s lab

GFP fusion

• GK192 (3610 ''lacA''::P''floT-yfp''(mls)), available in [SW|Daniel Lopez]'s lab
• JS136 (3610 ''amyE::floT''-gfp(spc)), available in [SW|Daniel Lopez]'s lab
• JS134 (3610 ''lacA::floA''-mEos2 (mls)), available in [SW|Daniel Lopez]'s lab
• JS167 (3610 ''lacA::floA''-PAmCherry (mls)), available in [SW|Daniel Lopez]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]) for FloA [Pubmed|25909364], available in [SW|Daniel Lopez]'s lab

References

Reviews

• 25652542

Original publications

• 16629676,11866510,18763711,20713508,22753055,22882210,23651456,22178969,24222488,25909364,26297017,27362352