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bshC [2017-12-14 12:20:47]
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bshC [2017-12-14 12:20:47]

cysteine-adding enzyme required for the synthesis of bacillithiol
locus
BSU15120
pI
5.25
mw
62.44 kDa
protein length
539 aa Sequence Blast
gene length
1617 bp Sequence Blast
function
biosynthesis of bacillithiol
product
cysteine-adding enzyme
essential
no
synonyms
yllA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.2|Biosynthesis of bacillithiol]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

    • Gene

    Coordinates
    1,578,376 → 1,579,995

    Phenotypes of a mutant

  • impaired growth in minimal medium, reduced activity of enzymes containing FeS cluster [Pubmed|25988368]
  • sensitivity to superoxide (paraquate) stress [Pubmed|25988368]

    • The protein

    Structure

  • [PDB|4WBD] [Pubmed|25496067]

    • Expression and Regulation

    Operons

    genes
    [gene|0F153803781C9AC8321FB14F926B645C45E53B00|ylbQ]-[gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]
    description
    [Pubmed|20308541]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|23894131], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

    • regulation

  • ''[protein|search|ylbQ]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
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    Biological materials

    Mutant

  • MGNA-B128 (bshC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1127 NBRP B. subtilis, Japan]
  • a ''bshC'' null mutant is available in [SW|John Helmann]s lab
  • BKE15120 (Δ[gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTAGAACTTCCTTT, downstream forward: _UP4_TGAAATAAAGTTTTAAAGAA
  • BKK15120 (Δ[gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTAGAACTTCCTTT, downstream forward: _UP4_TGAAATAAAGTTTTAAAGAA

    • References

    Reviews

  • 28117687

    • Original Publications

  • 25496067,8636036,20308541,19578333,23894131,25988368,29027347