bshC [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]

cysteine-adding enzyme required for the synthesis of bacillithiol

locus: BSU15120

pI: 5.00

mw: 62.00 kDa

function: biosynthesis of bacillithiol

product: cysteine-adding enzyme

essential: no

synonyms: yllA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.2|Biosynthesis of bacillithiol]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

[SW|Categories] containing this gene/protein

• [SW|miscellaneous metabolic pathways], [SW|resistance against oxidative and electrophile stress]

This gene is a member of the following [SW|regulons]

• [SW|Spx regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,578,376 -> 1,579,995

Phenotypes of a mutant

• impaired growth in minimal medium, reduced activity of enzymes containing FeS cluster [Pubmed|25988368]
• sensitivity to superoxide (paraquate) stress [Pubmed|25988368]

The protein

Structure

• [PDB|4WBD] [Pubmed|25496067]

Expression and Regulation

Operon

• ''[protein|search|ylbQ]-[protein|search|bshC]'' [Pubmed|20308541]

[SW|Sigma factor]

• ''[protein|search|ylbQ]'': [protein|search|SigA] [Pubmed|23894131]
• ''[protein|search|bshC]'': [protein|search|SigA] [Pubmed|23894131]

Regulation

• ''[protein|search|ylbQ]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]

Regulatory mechanism

• [protein|search|Spx]: transcription activation [Pubmed|23894131]

Biological materials

Mutant

• ''bshC'' null mutant available in [SW|John Helmann] lab

References

• 25496067,8636036,20308541,19578333,23894131,25988368