hom [2019-02-06 09:47:05]

homoserine dehydrogenase (NADPH)

locus: BSU32260

pI: 4.90

mw: 47.33 kDa

protein length: 433 aa

gene length: 1302 bp

function: biosynthesis of methionine and threonine

product: homoserine dehydrogenase (NADPH)

essential: no

ec: 1.1.1.3

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.6|Biosynthesis/ acquisition of lysine/ threonine]

Gene

Coordinates: 3,314,828 3,316,129

The protein

Catalyzed reaction/ biological activity

• L-homoserine + NAD(P)⁺ = L-aspartate 4-semialdehyde + NAD(P)H (according to Swiss-Prot)

Protein family

• homoserine dehydrogenase family (according to Swiss-Prot)

[SW|Domains]

• [SW|ACT domain] (aa 350 ... 426) (according to the Interpro database)

Effectors of protein activity

• subject to feedback inhibition [Pubmed|19258532]

Structure

• [PDB|2EJW] (from ''Thermus thermophilus hb8'', 37% identity, 57% similarity)

[SW|Localization]

• membrane associated [Pubmed|18763711]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|0F40291BB10DB6D7DFD94B9C7D700F4410C4EE69|yutH]-[gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]
  description: [Pubmed|22383849]

  regulation

  • expressed during [SW|sporulation] [Pubmed|22383849]

• genes: [gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]
  description: [Pubmed|12107147]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|24163341], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|25755103], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|A4B7CF7A1C704C750AFAD97A43AF975260935083|ThrR]: repression, [Pubmed|27260660], in [regulon|A4B7CF7A1C704C750AFAD97A43AF975260935083|ThrR regulon]

  regulation

  • expressed in the presence of lysine or cysteine ([SW|ThrR]) [Pubmed|27260660]

Biological materials

Mutant

• BKE32260 ([gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG
• BKK32260 ([gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG

lacZ fusion

• pBP312 (in [SW|pAC5]), available in [SW|Fabian Commichau]'s lab [Pubmed|27260660]

References

• 12107147,18763711,3139660,19258532,24163341,25777134,15378759,25755103,27260660