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sigL [Mon Jan 11 2016 11:34:01 GMT+0100 (CET)]
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sigL [Mon Jan 11 2016 11:34:01 GMT+0100 (CET)]

sigma factor of the RNA polymerase, Sigma-54, Sigma L
locus
BSU34200
pI
7.00
mw
49.00 kDa
function
utilization of arginin, acetoin and fructose,
required for cold adaptation
product
RNA polymerase sigma-54 factor (sigma-L)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]

    • [SW|Categories] containing this gene/protein

  • [SW|transcription], [SW|sigma factors and their control]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,512,498 -> 3,513,808

    Phenotypes of a mutant

  • The mutant is cold-sensitive and unable to use arginine as a single carbon source. [http://www.ncbi.nlm.nih.gov/sites/entrez/16585774 PubMed]

    • The protein

    Catalyzed reaction/ biological activity

  • Binding to promoters of the -12, -24 type

    • Protein family

  • sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family

    • [SW|Domains]

  • DNA binding domain (H-T-H motif) (324–343)
  • pron box domain (413–421)
  • 3 x Compositional bias domain (6–21),(32–53),(112–136)

    • Structure

  • [PDB|5BYH] (''E. coli'' [SW|RNA polymerase] containing Sigma-54) [Pubmed|26293966]

    • [SW|Interactions]

  • [protein|search|SigL]-([protein|search|RpoB]-[protein|search|RpoC]-[protein|search|RpoA]) [Pubmed|26293966]
  • [protein|search|SigL]-[protein|search|AcoR], [protein|search|SigL]-[protein|search|RocR], [protein|search|SigL]-[protein|search|LevR], [protein|search|SigL]-[protein|search|BkdR], [protein|search|SigL]-[protein|search|YplP],

    • Additional information

  • Transcription initiation by SigL-containing [SW|RNA polymerase] requires the activity of ATP-hydrolyzing transcription activators.
  • [SW|Transcription factors activating transcription at SigL-dependent promoters]

    • Expression and Regulation

    Operon

  • ''sigL'' (according to [http://dbtbs.hgc.jp/COG/prom/sigL.html DBTBS])

    • Regulation

  • repressed by glucose ([protein|search|CcpA]) [Pubmed|16166551]

    • Regulatory mechanism

  • [protein|search|CcpA]: transcription repression, transcriptional roadblock [Pubmed|16166551]

    • Biological materials

    Mutant

  • GP146 ( ''sigL''::spc), available in [SW|Jörg Stülke]'s lab [Pubmed|17183217]
  • 1A914 ( ''sigL''::''kan''), [Pubmed|16585774], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A914&Search=1A914 BGSC]

    • Labs working on this gene/protein

  • [SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]

    • References

    Reviews

  • 7934866,10894718,21054445,26010401,21906631

    • Original publications

  • 16585774,11274109,1924373,16166551,22900538,26293966,24553251