SubtiBank SubtiBank
leuB
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leuB

3-isopropylmalate dehydrogenase
Locus
BSU_28270
Isoelectric point
4.74
Molecular weight
39.79 kDa
Protein length
365 aa Sequence Blast
Gene length
1098 bp Sequence Blast
Function
biosynthesis of leucine
Product
3-isopropylmalate dehydrogenase
Essential
no
E.C.
1.1.1.85
Synonyms
leuC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of branched-chain amino acids
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

    • Gene

    Coordinates
    2,891,020 2,892,117

    The protein

    Catalyzed reaction/ biological activity

  • (2R,3S)-3-isopropylmalate + NAD+ --> 4-methyl-2-oxopentanoate + CO2 + NADH (according to UniProt)

    • Protein family

  • Isocitrate and isopropylmalate dehydrogenases family (with Icd and YcsA, according to UniProt)

    • Paralogous protein(s)

  • YcsA

    • Modification

  • phosphorylated on Arg-4 PubMed

    • Structure

  • 1XAD (Thermus thermophilus)

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • T-box: termination/antitermination, via tRNA controlled RNA switch, repression by BCAA, in T-box
  • TnrA: repression, PubMed, in TnrA regulon
  • CodY: repression, PubMed PubMed, in CodY regulon
  • CcpA: activation, PubMed, in CcpA regulon

    • Regulation

  • expression is stimulated in the presence of glucose (CcpA) PubMed

    • Additional information

  • An antisense RNA is predicted for leuA PubMed
  • the T-box RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • 1A618 ( leuB::erm), PubMed, available at BGSC
  • BKE28270 (leuB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGTTAACCGTTCTCCTTT, downstream forward: _UP4_TGACAGCTTACGTTAAGCGG
  • BKK28270 (leuB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGTTAACCGTTCTCCTTT, downstream forward: _UP4_TGACAGCTTACGTTAAGCGG

    • References

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