cspD

cold shock protein

Locus: BSU_21930

Isoelectric point: 4.31

Molecular weight: 7.17 kDa

Protein length: 66 aa

Gene length: 201 bp

Function: RNA chaperone

Product: cold shock protein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → RNA chaperones
• Lifestyles → Coping with stress → Cold stress proteins

Gene

Coordinates: 2,307,905 2,308,105

The protein

Paralogous protein(s)

• CspB, CspC

Domains

• CSD domain (aa 4-63) (according to UniProt)

Structure

• 1C9O (from B. caldolyticus, 86% identity) PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: cspD
  Description: PubMed

Biological materials

Mutant

• GP2614 (cspD::aphA3), available in Jörg Stülke's lab
• MGNA-A874 (cspD::erm), available at the NBRP B. subtilis, Japan
• BKE21930 (cspD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGCTTAATTCCTCCT, downstream forward: _UP4_TAAACTCAATACATGATGAT
• BKK21930 (cspD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGCTTAATTCCTCCT, downstream forward: _UP4_TAAACTCAATACATGATGAT

Expression vectors

• pGP2164: expression of cspD-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
• pGP2165: expression of cspD-Strep by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

Reviews

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Original publications

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