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leuD [Fri Apr 01 2016 13:41:04 GMT+0200 (CEST)]
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leuD [Fri Apr 01 2016 13:41:04 GMT+0200 (CEST)]

3-isopropylmalate dehydratase (small subunit)
locus
BSU28250
pI
4.00
mw
22.00 kDa
function
biosynthesis of leucine
product
3-isopropylmalate dehydratase (small subunit)
essential
no
ec
4.2.1.33
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|CodY regulon], [SW|FsrA regulon], [SW|T-box], [SW|TnrA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,888,940 -> 2,889,539

    The protein

    Catalyzed reaction/ biological activity

  • (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate H2O (according to Swiss-Prot)

    • Protein family

  • LeuD type 1 subfamily (according to Swiss-Prot)

    • [SW|Cofactors]

  • contains an iron-sulfur cluster

    • Structure

  • [PDB|2HCU] (from ''Streptococcus mutans'', 48% identity)

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=17981983 PubMed]

    • Expression and Regulation

    Operon

  • ''[protein|search|ilvB]-[protein|search|ilvH]-[protein|search|ilvC]-[protein|search|leuA]-[protein|search|leuB]-[protein|search|leuC]-[protein|search|leuD]'' [Pubmed|1577690]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|1577690]

    • Regulation

  • for a complete overview on the regulation of the ilv operon, see [http://www.ncbi.nlm.nih.gov/pubmed/20935095 Brinsmade ''et al''.]
  • repressed by casamino acids [Pubmed|12107147]
  • expression is stimulated in the presence of glucose [Pubmed|12193635]
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|15547269]
  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]
  • less expressed under conditions of extreme iron limitation ([protein|search|FsrA]) [Pubmed|18697947]

    • Regulatory mechanism

  • [protein|search|CodY]: transcription repression [Pubmed|12618455]
  • [protein|search|CcpA]: transcription activation [Pubmed|12193635]
  • repression by BCAA: tRNA-controlled [SW|RNA switch] ([protein|search|T-box]) that mediates termination/antitermination
  • [protein|search|TnrA]: transcription repression [Pubmed|15547269]
  • [protein|search|FsrA]: translational repression [Pubmed|18697947]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=17981983 PubMed]

    • References

  • 15060025,12193635,19258532,18697947,8289305,18641142,11948165,15547269,12618455,15547269,12618455,12107147,25157083,20935095,25755103,24163341,26220295