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sdhA
succinate dehydrogenase (flavoprotein subunit)
Molecular weight
65.17 kDa
Product
succinate dehydrogenase (flavoprotein subunit)
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
2,906,335 2,908,095
The protein
Catalyzed reaction/ biological activity
quinone + succinate --> quinol + fumarate (according to UniProt)Protein family
FAD-dependent oxidoreductase 2 family (with NadB, according to UniProt) Modification
Cofactors
FeFAD (according to UniProt)Effectors of protein activity
Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide PubMedActivated by Cytochrome b558 PubMed Structure
Localization
attached to the membrane PubMed Additional information
This enzyme is a membrane-bound trimer PubMed PubMedOne subunit is bound to cytochrome b558, and this subunit is the one bound to the cytosolic side of the membrane PubMed PubMedAnother subunit is the flavoprotein one, required for FAD usage PubMed PubMedThe other subunit has an iron-sulphur domain necessary for the catalytic activity PubMed PubMedextensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at Proteopedia Expression and Regulation
Biological materials
Mutant
GP743 ∆(sdhC-sdhA), cat, available in Jörg Stülke's labGP792 ∆(sdhC-sdhA-sdhB)::phleo, available in Jörg Stülke's labGP2342 ∆(sdhC-sdhA-sdhB)::kan, Cre-recombinase is integrated in sacA, available in Jörg Stülke's labGP2343 ∆(sdhC-sdhA-sdhB)::lox72, Cre-recombinase is integrated in sacA, available in Jörg Stülke's labBKE28440 ∆(sdhA)::erm trpC2 available at BGSC, PubMed, upstream reverse: _UP1_CATGATAGCCCCTCTCCCTC, downstream forward: _UP4_AAGAAGAAGGTGGCGAAATABKK28440 ∆(sdhA::kan) trpC2 available at BGSC, PubMed, upstream reverse: _UP1_CATGATAGCCCCTCTCCCTC, downstream forward: _UP4_AAGAAGAAGGTGGCGAAATA Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab References
Reviews
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Original publications
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