SubtiBank SubtiBank
sdhA
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sdhA

succinate dehydrogenase (flavoprotein subunit)
Locus
BSU_28440
Isoelectric point
5.71
Molecular weight
65.17 kDa
Protein length
586 aa Sequence Blast
Gene length
1761 bp Sequence Blast
Function
TCA cycle
Product
succinate dehydrogenase (flavoprotein subunit)
Essential
no
Synonyms
citF

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,906,335 2,908,095

The protein

Catalyzed reaction/ biological activity

  • quinone + succinate --> quinol + fumarate (according to UniProt)
  • Protein family

  • FAD-dependent oxidoreductase 2 family (with NadB, according to UniProt)
  • Modification

  • phosphorylated PubMed, PubMed
  • Cofactors

  • Fe
  • FAD (according to UniProt)
  • Effectors of protein activity

  • Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide PubMed
  • Activated by Cytochrome b558 PubMed
  • Structure

  • 1NEK (E. coli) PubMed
  • Localization

  • attached to the membrane PubMed
  • Additional information

  • This enzyme is a membrane-bound trimer PubMed PubMed
  • One subunit is bound to cytochrome b558, and this subunit is the one bound to the cytosolic side of the membrane PubMed PubMed
  • Another subunit is the flavoprotein one, required for FAD usage PubMed PubMed
  • The other subunit has an iron-sulphur domain necessary for the catalytic activity PubMed PubMed
  • extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at Proteopedia
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulation

  • constitutive
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    Other regulations

  • FsrA: translation inhibition, PubMed
  • Biological materials

    Mutant

  • GP743 ∆(sdhC-sdhA), cat, available in Jörg Stülke's lab
  • GP792 ∆(sdhC-sdhA-sdhB)::phleo, available in Jörg Stülke's lab
  • GP2342 ∆(sdhC-sdhA-sdhB)::kan, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • GP2343 ∆(sdhC-sdhA-sdhB)::lox72, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • BKE28440 ∆(sdhA)::erm trpC2 available at BGSC, PubMed, upstream reverse: _UP1_CATGATAGCCCCTCTCCCTC, downstream forward: _UP4_AAGAAGAAGGTGGCGAAATA
  • BKK28440 ∆(sdhA::kan) trpC2 available at BGSC, PubMed, upstream reverse: _UP1_CATGATAGCCCCTCTCCCTC, downstream forward: _UP4_AAGAAGAAGGTGGCGAAATA
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • References

    Reviews

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    Original publications

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