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lip [2019-07-22 10:35:45]
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lip [2019-07-22 10:35:45]

extracellular lipase
Locus
BSU_02700
Isoelectric point
10.06
Molecular weight
22.64 kDa
Protein length
212 aa Sequence Blast
Gene length
639 bp Sequence Blast
Function
lipid degradation
Product
extracellular lipase
Essential
no
Synonyms
lipA

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
292,205 292,843

The protein

Catalyzed reaction/ biological activity

  • triacylglycerol + H2O --> diacylglycerol + fatty acid + H+
  • Protein family

  • AB hydrolase superfamily (according to UniPort)
  • Paralogous protein(s)

    Structure

    Expression and Regulation

    Operons

    Genes
    Description

    Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
  • Regulation

  • repressed during vegetative growth (AbrB) PubMed
  • Additional information

  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed
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    Biological materials

    Mutant

  • BKE02700 (lip::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATATCCTCCTTTTTTT, downstream forward: _UP4_TAATGAAAAACAAAACCTTG
  • BKK02700 (lip::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATATCCTCCTTTTTTT, downstream forward: _UP4_TAATGAAAAACAAAACCTTG
  • References

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