iseA

inhibits in vitro activity of cell wall endopeptidases, inhibits cell separation

locus: BSU_18380

pI: 10.18

mw: 19.98 kDa

protein length: 181 aa

gene length: 546 bp

function: protection against cell envelope stress

product: inhibitor of autolysins

essential: no

synonyms: yoeB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,002,637 2,003,182

The protein

Structure

• [PDB|2RSX] [Pubmed|23091053]

[SW|Localization]

• localized to cell separation sites

Expression and Regulation

Operons

• genes: [gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: repression, [Pubmed|17581128], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]

  regulation

  • expression is modulated in response to D,L-endopeptidase activity (decreased upon reduced activity in the absence of [protein|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|CwlO] or [protein|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|FtsE]-[protein|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|FtsX], increased upon overexpression of [protein|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|LytE]) ([protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]) [pubmed|31808740]
  • induced by vancomycin [Pubmed|17483219]

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Mutant

• MGNA-B076 (yoeB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1075 NBRP B. subtilis, Japan]
• BKE18380 ([gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCGCTCTCTCCTTCA, downstream forward: _UP4_TAAGAAAAACGCCCGGTATA
• BKK18380 ([gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCGCTCTCTCCTTCA, downstream forward: _UP4_TAAGAAAAACGCCCGGTATA

References

• 17581128,18761694,17483219,20059685,22383849,23091053,23033921,23199363,26883633,29458655,29465029,31808740