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pgpB [2020-07-07 16:02:33]
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pgpB [2020-07-07 16:02:33]

phosphatidylglycerol phosphate (PGP) phosphatase , has minor undecaprenyl pyrophosphate phosphatase activity
Locus
BSU_19650
Isoelectric point
10.14
Molecular weight
22.64 kDa
Protein length
203 aa Sequence Blast
Gene length
612 bp Sequence Blast
Function
phospholipid biosynthesis
Product
phosphatidylglycerol phosphate (PGP) phosphatase
Essential
no
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of phospholipids
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,136,913 2,137,524

    The protein

    Catalyzed reaction/ biological activity

  • 1,2-diacyl-sn-glycero-3-phospho-(1ʼ-sn-glycero-3ʼ-phosphate) + H2O --> 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate (according to UniProt)

    • Protein family

  • PA-phosphatase related phosphoesterase family (single member, according to UniProt)

    • Structure

  • 5JKI PubMed

    • Localization

  • cell membrane (integral) PubMed

    • Expression and Regulation

    Additional information

  • the mRNA is cleaved by RNase III PubMed

    • Biological materials

    Mutant

  • MGNA-B441 (pgpB::erm), available at the NBRP B. subtilis, Japan
  • BKE19650 (pgpB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC
  • BKK19650 (pgpB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC
  • GP3652 ΔpgpB::cat, available in Jörg Stülke's lab

    • Expression vector

  • pGP3506 (N-terminal His-tag, expression in E. coli, in pWH844), available in Jörg Stülke's lab

    • References

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