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aprE [Wed Jun 15 2016 16:25:06 GMT+0200 (CEST)]
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aprE [Wed Jun 15 2016 16:25:06 GMT+0200 (CEST)]

major extracellular alkaline protease
Locus
BSU10300
Isoelectric point
9.00
Molecular weight
39.00 kDa
Function
protein degradation
Product
extracellular alkaline serine protease (subtilisin E))
Essential
no
Synonyms
sprE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of proteins
  • Information processing → Protein synthesis, modification and degradation → Proteolysis → Extracellular feeding proteases
  • Groups of genes → Secreted proteins

    • Categories containing this gene/protein

  • utilization of nitrogen sources other than amino acids, proteolysis

    • This gene is a member of the following regulons

  • AbrB regulon, CodY regulon, DegU regulon, ScoC regulon, SinR regulon

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,104,423 -> 1,105,568

    The protein

    Catalyzed reaction/ biological activity

  • Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1 (according to Swiss-Prot)

    • Protein family

  • peptidase S8 family (according to Swiss-Prot)

    • Structure

  • 1SBC

    • Localization

  • secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

    • Interactions

  • AprE-PaiA PubMed

    • Expression and Regulation

    Operon

  • aprE (according to DBTBS)

    • Sigma factor

  • SigA PubMed

    • Regulation

  • repressed by SinR PubMed
  • repressed during exponential growth (ScoC, AbrB) PubMed

    • Regulatory mechanism

  • SinR: transcription repression PubMed
  • ScoC: transcription repression PubMed
  • AbrB: transcription repression PubMed
  • DegU-P: transcription activation PubMed
  • CodY: transcription repression PubMed

    • Additional information

  • in a triple abrB codY scoC mutant, expression occurs during exponential growth PubMed
  • the mRNA is extremely stable (more than 25 min) [Pubmed|11101663 ]

    • Biological materials

    Antibody

  • **

    • References

    Reviews

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    Original publications

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