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tpi [Wed Oct 28 2015 18:27:12 GMT+0100 (CET)]
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tpi [Wed Oct 28 2015 18:27:12 GMT+0100 (CET)]

triose phosphate isomerase, glycolytic/ gluconeogenic enzyme
locus
BSU33920
pI
4.00
mw
26.00 kDa
function
enzyme in glycolysis/ gluconeogenesis
product
triosephosphate isomerase
essential
no
ec
5.3.1.1
synonyms
tpiA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CggR regulon]

    • Gene

    Phenotypes of a mutant

  • essential [Pubmed|12682299], non-essential according to [Pubmed|23420519]

    • The protein

    Catalyzed reaction/ biological activity

  • D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate (according to Swiss-Prot)

    • Protein family

  • triosephosphate isomerase family (according to Swiss-Prot)

    • Modification

  • phosphorylation on Ser-213 [Pubmed|17218307]

    • Effectors of protein activity

  • inhibited by 2-phosphoglycolate (in ''B. stearothermophilus'') [Pubmed|8580851]

    • Structure

  • [PDB|1BTM] (complex with 2-phosphoglycolic acid, Geobacillus stearothermophilus), complex with 2-phosphpoglycolic acid, ''Geobacillus stearothermophilus'' [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=48255 NCBI]

    • [SW|Localization]

  • cytoplasm (homogeneously distributed throughout the cell) [Pubmed|24825009,16479537]

    • [SW|Interactions]

  • [protein|search|Tkt]-[protein|search|Tpi] [Pubmed|19193632]

    • Additional information

  • extensive information on the structure and enzymatic properties of Tpi can be found at [http://www.proteopedia.org/wiki/index.php/Triose_Phosphate_Isomerase Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|cggR]-[protein|search|gapA]-[protein|search|pgk]-[protein|search|tpiA]-[protein|search|pgm]-[protein|search|eno]'' [Pubmed|11489127]
  • ''[protein|search|pgk]-[protein|search|tpiA]-[protein|search|pgm]-[protein|search|eno]'' [Pubmed|11489127]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|11489127]

    • Regulation

  • expression activated by glucose (3.3 fold) [Pubmed|12850135]
  • ''[protein|search|cggR]'': induced by glycolytic substrates [protein|search|CggR] [Pubmed|11489127]
  • ''[protein|search|pgk]'': constitutive [Pubmed|11489127]

    • Regulatory mechanism

  • transcription repression by [protein|search|CggR] [Pubmed|11489127]

    • Additional information

  • belongs to the 100 [[most abundant proteins]] [PubMed|15378759]

    • Biological materials

    Mutant

  • GP700 (cat), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]

    • Expression vector

  • pGP394 (N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP89 (N-terminal Strep-tag, for [SW|SPINE], expression in B. subtilis), available in [SW|Jörg Stülke]'s lab
  • pGP1511 (expression in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

  • 12850135,11489127,17505547,8021172,17218307,8580851,19193632,21821766,23420519,15378759,24825009