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murQ [2019-06-17 12:32:36]
N-acetyl-muramic acid-6P etherase (cleaves lactate from N-acetylmuramic acid)
function
cell wall turnover
product
N-acetyl-muramic acid-6P etherase
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.3|Utilization of cell wall components][category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.17|Utilization of amino sugars][category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.3|Utilization of amino sugars][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]Gene
Coordinates
192,051 192,965
Phenotypes of a mutant
strongly reduced viability during prolonged incubation in stationary phase [Pubmed|27729505]The protein
Catalyzed reaction/ biological activity
N-acetylmuramic acid 6-phosphate + H2O = N-acetyl-D-glucosamine 6-phosphate + D-lactate (according to Swiss-Prot)Protein family
SIS domain (according to Swiss-Prot)Modification
phosphorylation on Ser-2 [Pubmed|17218307]Structure
[PDB|4S12] (from Yersinia enterocolitica, 51% identity)Expression and Regulation
Operons
genes
[gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]-[gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]-[gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]-[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]-[gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]-[gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|ybbC]
description
[Pubmed|22383849]
regulatory mechanism
[protein|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR]: repression, [pubmed|30038046], in [regulon|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR regulon]regulation
expressed in late exponential and early stationary phase [Pubmed|20400549]view in new tabBiological materials
Mutant
MGNA-B952 (ybbI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1951 NBRP B. subtilis, Japan]BKE01700 ([gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGGATGCCCCCTGTTT, downstream forward: _UP4_CATCCATGATAAGGAGAGAABKK01700 ([gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGGATGCCCCCTGTTT, downstream forward: _UP4_CATCCATGATAAGGAGAGAAReferences
17218307,20400549,22383849,27729505,30038046