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murQ [2019-08-26 12:55:19]
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murQ [2019-08-26 12:55:19]

N-acetyl-muramic acid-6P etherase (cleaves lactate from N-acetylmuramic acid)
locus
BSU_01700
pI
5.40
mw
32.55 kDa
protein length
304 aa Sequence Blast
gene length
915 bp Sequence Blast
function
cell wall turnover
product
N-acetyl-muramic acid-6P etherase
essential
no
synonyms
ybbI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.3|Utilization of cell wall components]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.17|Utilization of amino sugars]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.3|Utilization of amino sugars]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    192,051 192,965

    Phenotypes of a mutant

  • strongly reduced viability during prolonged incubation in stationary phase [Pubmed|27729505]

    • The protein

    Catalyzed reaction/ biological activity

  • H2O + N-acetyl-D-muramate 6-phosphate --> (R)-lactate + N-acetyl-D-glucosamine 6-phosphate (according to UniProt)

    • Protein family

  • GCKR-like family (single member, according to UniProt)

    • [SW|Domains]

  • [SW|SIS domain] (aa 59-122) (according to UniProt)

    • Modification

  • phosphorylation on Ser-2 [Pubmed|17218307]

    • Structure

  • [PDB|4S12] (from Yersinia enterocolitica, 51% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]-[gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]-[gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]-[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]-[gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]-[gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|ybbC]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR]: repression, [pubmed|30038046], in [regulon|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR regulon]

    • regulation

  • expressed in late exponential and early stationary phase [Pubmed|20400549]
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    Biological materials

    Mutant

  • MGNA-B952 (ybbI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1951 NBRP B. subtilis, Japan]
  • BKE01700 ([gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGGATGCCCCCTGTTT, downstream forward: _UP4_CATCCATGATAAGGAGAGAA
  • BKK01700 ([gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGGATGCCCCCTGTTT, downstream forward: _UP4_CATCCATGATAAGGAGAGAA

    • References

  • 17218307,20400549,22383849,27729505,30038046