yqiI

secreted N-acetylmuramoyl-L-alanine amidase

Locus: BSU_24190

Isoelectric point: 4.29

Molecular weight: 22.09 kDa

Protein length: 206 aa

Gene length: 621 bp

Function: cell wall metabolism

Product: peptidoglycan hydrolase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Utilization of cell wall components
• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Cell wall degradation/ turnover/ Additional genes

Gene

Coordinates: 2,514,979 2,515,599

The protein

Catalyzed reaction/ biological activity

• cell wall hydrolysis PubMed

Protein family

• N-acetylmuramoyl-L-alanine amidase 3 family (according to UniProt)

Domains

• contains an amidase_3 domain (like CwlC, CwlD, LytC, YrvJ)
• MurNAc-LAA domain (aa 32-201) (according to UniProt)

Structure

• 4RN7 (from Clostridium difficile, 39% identity)

Localization

• secreted (with N-terminal signal sequence for SEC-mediated transport) PubMed

Expression and Regulation

Operons

• Genes: yqiH-yqiI-yqiK
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulation

  • only expressed under conditions of high osmolarity PubMed

Biological materials

Mutant

• MGNA-C372 (yqiI::erm), available at the NBRP B. subtilis, Japan
• BKE24190 (yqiI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCAACTTACTCCCTC, downstream forward: _UP4_TAGATTGATACAGCCTTAAT
• BKK24190 (yqiI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCAACTTACTCCCTC, downstream forward: _UP4_TAGATTGATACAGCCTTAAT

References

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