yqiI

secreted N-acetylmuramoyl-L-alanine amidase

locus: BSU_24190

pI: 4.29

mw: 22.09 kDa

protein length: 206 aa

gene length: 621 bp

function: cell wall metabolism

product: peptidoglycan hydrolase

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.3|Utilization of cell wall components]
• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.6|Cell wall degradation/ turnover/ Additional genes]

Gene

Coordinates: 2,514,979 2,515,599

The protein

Catalyzed reaction/ biological activity

• cell wall hydrolysis [Pubmed|22843846]

Protein family

• [SW|N-acetylmuramoyl-L-alanine amidase 3 family] (according to UniProt)

[SW|Domains]

• contains an amidase_3 domain (like [protein|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|CwlC], [protein|B09FB626274B81F00A4FB42D188E089095343182|CwlD], [protein|6A21293823151C6980BF52B31A4B249A8440F2E1|LytC], [protein|BE34A9CE3AF99E8880FC24E34E5D79A1627260AE|YrvJ])
• [SW|MurNAc-LAA domain] (aa 32-201) (according to UniProt)

Structure

• [PDB|4RN7] (from Clostridium difficile, 39% identity)

[SW|Localization]

• secreted (with N-terminal signal sequence for SEC-mediated transport) [Pubmed|22843846]

Expression and Regulation

Operons

• genes: [gene|AEA18C431FE13FC34671E673DC6733B200E342A8|yqiH]-[gene|0AD75864794E597AA9160969ADE6FE0C7ED07B9F|yqiI]-[gene|AF37A0E08AAAA9159DD1F0183A61713EF582E830|yqiK]
  description: [Pubmed|22843846,22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22843846], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • only expressed under conditions of high osmolarity [Pubmed|32419322,22843846]

Biological materials

Mutant

• MGNA-C372 (yqiI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2370 NBRP B. subtilis, Japan]
• BKE24190 ([gene|0AD75864794E597AA9160969ADE6FE0C7ED07B9F|yqiI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCAACTTACTCCCTC, downstream forward: _UP4_TAGATTGATACAGCCTTAAT
• BKK24190 ([gene|0AD75864794E597AA9160969ADE6FE0C7ED07B9F|yqiI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCAACTTACTCCCTC, downstream forward: _UP4_TAGATTGATACAGCCTTAAT

References

• 22383849,22843846,32419322