SubtiBank SubtiBank
yqiI
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yqiI

secreted N-acetylmuramoyl-L-alanine amidase
Locus
BSU_24190
Isoelectric point
4.29
Molecular weight
22.09 kDa
Protein length
206 aa Sequence Blast
Gene length
621 bp Sequence Blast
Function
cell wall metabolism
Product
peptidoglycan hydrolase
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,514,979 2,515,599

The protein

Catalyzed reaction/ biological activity

  • cell wall hydrolysis PubMed
  • Protein family

    Domains

  • contains an amidase_3 domain (like CwlC, CwlD, LytC, YrvJ)
  • MurNAc-LAA domain (aa 32-201) (according to UniProt)
  • Structure

  • 4RN7 (from Clostridium difficile, 39% identity)
  • Localization

  • secreted (with N-terminal signal sequence for SEC-mediated transport) PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulation

  • only expressed under conditions of high osmolarity PubMed
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    Biological materials

    Mutant

  • MGNA-C372 (yqiI::erm), available at the NBRP B. subtilis, Japan
  • BKE24190 (yqiI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCAACTTACTCCCTC, downstream forward: _UP4_TAGATTGATACAGCCTTAAT
  • BKK24190 (yqiI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCAACTTACTCCCTC, downstream forward: _UP4_TAGATTGATACAGCCTTAAT
  • References

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