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pheS
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pheS

phenylalanyl-tRNA synthetase (alpha subunit), universally conserved protein
locus
BSU_28640
pI
5.08
mw
38.52 kDa
protein length
344 aa Sequence Blast
gene length
1035 bp Sequence Blast
function
translation
product
phenylalanyl-tRNA synthetase (alpha subunit)
essential
yes
ec
6.1.1.20
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]

    • Gene

    Coordinates
    2,929,438 2,930,472

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + L-phenylalanine + tRNAPhe --> AMP + diphosphate + H+ + L-phenylalanyl-tRNAPhe (according to UniProt)

    • Protein family

  • [SW|Class-II aminoacyl-tRNA synthetase family] (according to UniProt)

    • Structure

  • [PDB|1PYS] (the [protein|0ABAEC20F55970C7C1EC5FFE197115E401BF03E8|PheS]-[protein|B255B87EB7A850D1C6B7930A3731DAD6AE0A107D|PheT] complex from ''Thermus thermophilus hb8 mutant'', 37% identity, 56% similarity) [Pubmed|7664121]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Additional information

  • [SW|universally conserved protein]
  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|0ABAEC20F55970C7C1EC5FFE197115E401BF03E8|pheS]-[gene|B255B87EB7A850D1C6B7930A3731DAD6AE0A107D|pheT]
    description
    [Pubmed|2127701]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2127701], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|T-box|T-box]: transcriptional antitermination, via tRNA controlled [SW|RNA switch], in [regulon|T-box|T-box]

    • regulation

  • induced by phenylalanine limitation ([SW|T-box]) [Pubmed|19258532]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
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    References

  • 19258532,2127701,15378759,28516784,29794222