yxkO [2019-08-12 13:26:33]

ADP/ATP-dependent NAD(P)H-hydrate dehydratase, general stress protein, survival of ethanol stress

locus: BSU_38720

pI: 5.49

mw: 29.72 kDa

protein length: 276 aa

gene length: 831 bp

function: survival of ethanol stress, repair of hydrated NAD(P)H

product: ADP/ATP-dependent NAD(P)H-hydrate dehydratase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

Gene

Coordinates: 3,972,448 3,973,278

The protein

Protein family

• NnrD/CARKD family (single member, according to UniProt)

Structure

• [PDB|1KYH] [pubmed|12457846]

Expression and Regulation

Operons

• genes: [gene|0A9C77804C09CD5E7D1D5D274E01B5B14920A1A9|yxkO]
  description: [Pubmed|10746760]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

Biological materials

Mutant

• MGNA-B749 (yxkO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1748 NBRP B. subtilis, Japan]
• BKE38720 ([gene|0A9C77804C09CD5E7D1D5D274E01B5B14920A1A9|yxkO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTATCCCTCCTCTGC, downstream forward: _UP4_TTTGAATGATAAAAAAGCTG
• BKK38720 ([gene|0A9C77804C09CD5E7D1D5D274E01B5B14920A1A9|yxkO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTATCCCTCCTCTGC, downstream forward: _UP4_TTTGAATGATAAAAAAGCTG

References

• 15805528,10746760,12457846,22940582,25393291,17702456