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yxkO
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yxkO

ADP/ATP-dependent NAD(P)H-hydrate dehydratase, general stress protein, survival of ethanol stress
Locus
BSU_38720
Isoelectric point
5.49
Molecular weight
29.72 kDa
Protein length
276 aa Sequence Blast
Gene length
831 bp Sequence Blast
Function
survival of ethanol stress, repair of hydrated NAD(P)H
Product
ADP/ATP-dependent NAD(P)H-hydrate dehydratase
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Coping with stress → General stress proteins (controlled by SigB)

    • Gene

    Coordinates
    3,972,448 3,973,278

    The protein

    Catalyzed reaction/ biological activity

  • (6S)-NADHX + ADP --> AMP + H+ + NADH + phosphate (according to UniProt)
  • (6S)-NADPHX + ADP --> AMP + H+ + NADPH + phosphate (according to UniProt)

    • Protein family

  • NnrD/CARKD family (single member, according to UniProt)

    • Domains

  • YjeF C-terminal domain (aa 7-275) (according to UniProt)

    • Structure

  • 1KYH PubMed

    • Expression and Regulation

    Operons

    Genes
    yxkO
    Description
    PubMed

    Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

    • Regulation

  • induced by stress (SigB) PubMed
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    Biological materials

    Mutant

  • MGNA-B749 (yxkO::erm), available at the NBRP B. subtilis, Japan
  • BKE38720 (yxkO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCTATCCCTCCTCTGC, downstream forward: _UP4_TTTGAATGATAAAAAAGCTG
  • BKK38720 (yxkO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCTATCCCTCCTCTGC, downstream forward: _UP4_TTTGAATGATAAAAAAGCTG

    • References

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