miaA [2017-8-11 16:0:3]

tRNA isopentenylpyrophosphate transferase

locus: BSU17330

pI: 6.00

mw: 35.52 kDa

protein length: 314 aa

gene length: 942 bp

function: tRNA modification

product: tRNA isopentenylpyrophosphate transferase

essential: no

ec: 2.5.1.8

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 1,866,389 → 1,867,333

Phenotypes of a mutant

• inactivation of ''[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]'' reduces sporulation efficiency to 16% that of wild type cells [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine (according to Swiss-Prot)

Protein family

• IPP transferase family (according to Swiss-Prot)

Structure

• [PDB|2QGN] (from ''Bacillus halodurans'', 57% identity)

Expression and Regulation

Operons

• genes: [gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
  description: [pubmed|22383849]

• genes: [gene|61B511C49912F1380AEE18AD300255A435F56F94|ymaF]-[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
  description: [pubmed|22383849]

  regulation

  • expressed during [SW|sporulation ][Pubmed|22383849]

References

The corresponding gene/ protein in other bacteria

• 11160115,3045085,9294434,26735940